| 人类染色体8q24.1带特异性微小卫星DNA的筛选 |
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| 引用本文： | 徐磊.人类染色体8q24.1带特异性微小卫星DNA的筛选[J].遗传学报,1997,24(1):1-6. |
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| 作者姓名： | 徐磊 |
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| 作者单位： | 医学遗传学国家重点实验室湖南医科大学 |
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| 摘 要： | 本研究运用人类高分辨染色体显微切割、ＰＣＲ技术获得的８ｑ２４．１带特异性探针池，构建了该区带的ｐＵＣ１９文库，从中筛选出４８个含ＣＡ重复顺序的微小卫星ＤＮＡ的克隆，已完成１２个克隆的序列分析，发现了一个世界上至今未曾报道过的、在正常人群中已检出１１个等位片段的、杂合度在中国汉族人群和美国盎格鲁撤克逊族人群中分别达０．８４和０．８３的高度多态的微小卫星ＤＮＡ（编号：Ｄ８Ｓ７Ｆ），经ＰＣＲ检测人鼠杂种细胞系列，证实其来源于人８号染色体。
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| 关 键 词： | 微小卫星DNA，（CA）_n，区带特异性，pUC19文库 |
Isolation of Band-specific(CA) n Microsatellites from Human Chromosome 8q24 1 |
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| XU Lei,XIA Jiahui,PAN Qian,CAI Tao,DENG Hanxiang.Isolation of Band-specific(CA) n Microsatellites from Human Chromosome 8q24 1[J].Journal of Genetics and Genomics,1997,24(1):1-6. |
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| Authors: | XU Lei XIA Jiahui PAN Qian CAI Tao DENG Hanxiang |
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| Abstract: | Fourty eight recombinant plasmids containing CA repeats were isolated from human chromosome 8q24 1 band-specific pUC19 library Among them,inserted DNA fragments from 12 plasmids were sequenced,and one new highly polymorphic(CA) n microsatellite was obtained(coding:D8S7F) It contained 11 alleles The estimated heterozygosity reached 0 84 and 0 83 among Chinese Han Nationality people and American Anglo-Saxons respectively It was assigned to chromosome 8 by using a human-rodent hybrid cell panel |
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| Keywords: | Microsatellite DNA (CA) n Chromosome band-specific pUC19 library |
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