Molecular weights from approach-to-sedimentation equilibrium data using nonlinear regression analysis |
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Authors: | Holladay L A |
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Institution: | Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232, USA. |
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Abstract: | Data obtained from early times during the transient period of sedimentation equilibrium experiments are analyzed using an approximate solution to the Lamm equation to estimate s/D. The Cr versus r data obtained at several times during approach-to-equilibrium are analyzed using a nonlinear least squares algorithm and Fujita's approximate solution. This procedure was tested using D-Ser13-somatostatin, ribonuclease, and ovalbumin. The results obtained demonstrate that for monodisperse samples s/D may be rapidly and reliably estimated using this method. |
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