水稻受盐抑制基因OsZFP1的转基因分析

OsZFP1(水稻锌指蛋白1)基因编码的蛋白含有3个推测的Cys2/Cys2-型锌指结构域,它的表达受盐胁迫负调控.构建了以35S为启动子的OsZFP1基因的植物表达载体,并将其转入拟南芥(Arabidopsis thaliana L.)植物和水稻(Oryza sativa L.)愈伤组织中以过量表达OsZFP1基因.转基因的拟南芥植株和水稻愈伤组织对盐处理的敏感性都比野生型要高.这一结果表明OsZFP1基因可能编码一种负调控蛋白,它可能抑制某些盐诱导基因的表达.在ABA处理下,转基因拟南芥植株比野生型植株抽苔晚,说明OsZFP1基因的作用可能受ABA调节.

Transgenic Analysis of a Salt-inhibited OsZFP1 Gene from Rice

OsZFP1 (Oryza sativa zinc finger protein No.1) gene encodes a protein containing three putative Cys₂/Cys₂-type zinc-finger domains, and is negatively regulated by salt stress. The plant expres-sion vector carrying the OsZFP1 gene was constructed under the control of 35S promoter in pCAMBIA1300, and then transformed into Arabidopsis plants and rice calli to overexpress OsZFP1 gene. Both the transgenic Arabidopsis plants and the transgenic rice calli were more sensitive to salt treatment than the wild type. This result implies that OsZFP1 gene may function as a repressor, which probably inhibits the expression of some salt-inducible genes. The transgenic Arabidopsis plants also bolted later than wild type plants upon ABA treatment, suggesting that the function of the OsZFP1 gene may be regulated by ABA.