Plant regeneration <Emphasis Type="Italic">in vitro</Emphasis> directly from cotyledon and hypocotyl explants of <Emphasis Type="Italic">Perilla frutescens</Emphasis> and their morphological aspects |
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Authors: | T?Zhang Email author" target="_blank">X?Y?WangEmail author Z?Y?Cao |
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Institution: | (1) The Institute of Cell Biology, Lanzhou University, Lanzhou, 730000, P.R. China;(2) Gansu Academy of Agricultural Science, Lanzhou, 730070, P.R. China;(3) School of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070, P.R. China |
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Abstract: | A rapid plantlet regeneration system for Perilla frutescens was established from cotyledon and hypocotyl explants. A maximum of 91.06 % cotyledon and 76.4 % hypocotyl explants could directly produce shoots (3.09 ± 0.18 shoots per explants) on Murashige and Skoog (MS) medium. The optimum hormone combinations were 4.44 μM 6-benzylaminopurine (BA) for cotyledon and 2.22 μM BA + 2.85 μM indole-3-acetic acid (IAA) for hypocotyls. Rooting was induced on half-strength hormone-free MS medium. After transplantation to soil, approximate 80 % of the regenerated plantlets could survive, flower and fruit. Moreover, some morphological abnormalities were found among the regenerated plants. |
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Keywords: | growth regulators direct organogenesis morphological abnormalities |
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