Analysis of the transgenic tobacco plants expressing Panicum miliaceum aspartate aminotransferase genes |
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| Authors: | N Sentoku M Taniguchi T Sugiyama K Ishimaru R Ohsugi F Takaiwa S Toki |
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| Institution: | (1) Nagoya University, BioScience Center, Chikusa, Nagoya 464–8601, Japan, JP;(2) Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Nagoya 464–8601, Japan, JP;(3) National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305–8602 Japan e-mail: stoki@abr.affrc.go.jp Fax: +81–298–388397, JP |
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| Abstract: | Expression of Panicum miliaceum L. (proso millet) mitochondrial and cytosolic aspartate aminotransferase (mAspAT and cAspAT, respectively) genes in transgenic
tobacco plants (Nicotiana tabacum) and their influences on protein synthesis were examined. The mAspAT- or cAspAT-transformed plants had about threefold or
3.5-fold higher AspAT activity in the leaf than non-transformed plants, respectively. Interestingly, the leaves of both transformed
plants had increased levels of phosphoenolpyruvate carboxylase (PEPC) and transformed plants with cAspAT also had increased levels of mAspAT in the leaf. These results
suggest that the increased expression of Panicum cAspAT in transgenic tobacco enhances the expression of its endogenous mAspAT and PEPC, and the increased expression of Panicum mAspAT enhances the expression of its endogenous PEPC.
Received: 6 February 1998 / Revision received: 6 August 1999 · Accepted: 6 September 1999 |
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| Keywords: | Aspartate aminotransferase Phosphoenolpyruvate carboxylase Isoenzyme Transgenic tobacco |
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