Neutral proteases of the genus Bacillus |
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| Authors: | L Keay |
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| Affiliation: | 1. NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China;2. Department of Blood Transfusion, the Affiliated Hospital of Qingdao University, Qingdao, Shandong, China |
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| Abstract: | Neutral protease of B. subtilis NRRL B3411 has been highly purified by chromatography on hydroxylapatite, and compared with B. subtilis var. amylosacchariticus neutral protease and thermolysin. On the basis of activity against macromolecular substrates no differentiation was possible. Immunochemical and stability studies as well as amino acid composition distinguished thermolysin from the other proteases which were indistinguishable. |
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