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Proteins reacting with anti-spectrin antibodies are present in Chlamydomonas Cells. |
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| Authors: | Michal Lorenz Brygida Bisikirska Beata Hanus-Lorenz Kazimierz Strzalka Aleksander F Sikorski |
| Institution: | The University of Queensland, Institute for Molecular Bioscience, 306 Carmody Road, St Lucia, Australia;Department of Ecogenomics and Systems Biology, Faculty of Life Sciences, University of Vienna, Vienna, Austria;Department of Psychiatry and Surgery (Transplant), Yale University, New Haven, CT, United States |
| Abstract: | It was found either in Western-blot analysis or in indirect immunofluorescence microscopy that cells of the alga Chlamydomonas reinhardhi contain polypeptides cross-reactng with antibodies directed against red blood cell spectrin. The protein could also be detected by immunoprecipitation with anti-spectrin antibodies. C. reinhardtii cells contain distinct polypeptide chains reacting with antibodies directed against either α- or β- spectrin subunits. This protein was extracted from the cells with low ionic strength solution but was not with nonionic detergent. |
| Keywords: | spectrin spectrin-like proteins membrane skeleton Chlamydomonas reinhardtii |
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