Development and evaluation of single-nucleotide polymorphism markers in allotetraploid rapeseed (<Emphasis Type="Italic">Brassica napus</Emphasis> L.) |
| |
Authors: | Peter Westermeier Gerhard Wenzel Volker Mohler |
| |
Institution: | 1.Plant Breeding, Department of Plant Sciences,Center of Life and Food Sciences Weihenstephan, Technische Universit?t München,Freising,Germany;2.Dean’s Office, Center of Life and Food Sciences Weihenstephan,Technische Universit?t München,Freising,Germany;3.Institute for Crop Science and Plant Breeding,Bavarian State Research Center for Agriculture,Freising,Germany |
| |
Abstract: | Single-nucleotide polymorphisms (SNPs) and insertion–deletions (INDELs) are currently the important classes of genetic markers
for major crop species. In this study, methods for developing SNP markers in rapeseed (Brassica napus L.) and their in silico mapping and use for genotyping are demonstrated. For the development of SNP and INDEL markers, 181
fragments from 121 different gene sequences spanning 86 kb were examined. A combination of different selection methods (genome-specific
amplification, hetero-duplex analysis and sequence analysis) allowed the detection of 18 singular fragments that showed a
total of 87 SNPs and 6 INDELs between 6 different rapeseed varieties. The average frequency of sequence polymorphism was estimated
to be one SNP every 247 bp and one INDEL every 3,583 bp. Most SNPs and INDELs were found in non-coding regions. Polymorphism
information content values for SNP markers ranged between 0.02 and 0.50 in a set of 86 varieties. Using comparative genetics
data for B. napus and Arabidopsis thaliana, an allocation of SNP markers to linkage groups in rapeseed was achieved: a unique location was determined for seven gene
sequences; two and three possible locations were found for six and four sequences, respectively. The results demonstrate the
usefulness of existing genomic resources for SNP discovery in rapeseed. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|