SCGE技术检测镉对背角无齿蚌血细胞DNA的损伤

以背角无齿蚌(Anodonta woodiana woodiana)为研究对象,利用单细胞凝胶电泳技术检测不同浓度及不同染毒时间氯化镉对背角无齿蚌血细胞DNA的损伤作用。结果显示,染毒后的背角无齿蚌血细胞均出现了拖尾现象,各染毒组与对照组相比,拖尾增长明显。剂量效应组中,蚌暴露在不同浓度(0、1、10、50mg·L^-1)的氯化镉中72 h,各组蚌血细胞DNA平均迁移长度及彗尾DNA含量均明显增加,与阴性对照组比较差异显著(p<0.01),存在显著的剂量-效应关系;在时间效应组中,蚌暴露在10mg·L^-1的氯化镉中分别24、48、72、96 h,随着氯化镉染毒时间的延长,各染毒组细胞DNA平均迁移长度及彗尾DNA含量与0时间组比较差异显著(p<0.01),但时间-效应关系不明显。

Detection of DNA Damages in Hemocytesof A.woodiana woodiana Induced by Cadmium with single Cell Gel electrophoresis Assay

In this study,mussels(Anodonta woodiana woodiana) were chosen as the research object.We assessed the possible DNA damage in haemocytes of mussels using the single cell gel electrophoresisassay.Results showed haemocytes images which exposed to cadimium presented comet tails,compared the exposed groups with negative control group,existed significant differences.Mussels were exposed to different cadmium chloride concentrations(0,1,10,50 mg·L^-1) respectively for 72 hours,the results showed that the average length of DNA migration and tai lDNA% in all exposed groups was significantly higher than that of negative control group(p<0.01) and existed significant dose-effect relationship.Otherwise,at the concentration of 10 mg·L^-1,four time-effect groups(24,48,72,96 h) and a zero time control group were set up,the average length of DNA migration and tail DNA% under different exposed times in cadmium chloride was significantly higher than zero time group(p<0.01),but no significant time-effect relationship among the tested concentrations.