长链烷烃降解菌的降解特性

对长链烷烃降解菌的降解能力和摄取模式进行了研究。评价14株烃降解菌利用中长链烃生长的能力,发现只有少数烃降解菌能够获得良好生长,其中Mycobacterium fortuitum514,Pseudomonas aeruginosa1785和Pseudomonas marginata766等3株菌能够高效降解C20到C33的长链烷烃。辛烷不能支持这些长链烷烃降解菌的生长,说明其烃氧化酶与Pseudomonas oleovorans的OCT质粒编码的单氧酶不同。此外,M.fortuitum不产胞外表面活性剂,而P.aeruginosa和P.marginata则是表面活性剂产生菌,然而三者在以烃为碳源生长时均显示出很高的细胞表面疏水性。根据生长现象分析3株菌采用了不同的烷烃摄取模式。

Degradation Characteristics of Long-chain Aliphatic Alkanedegrading Bacteria

The degradation ability and uptake modes of Long-chain alkane-degrading bacteria were investigated.Among 14 bacterial species belonging to hydrocarbon-degrading bacteria,only a few strains were capable of utilizing long chain alkanes as the sole carbon source.Mycobacterium fortuitum 514,Pseudomonas aeruginosa 1785 and Pseudomonas marginata 766 showed the exceptional capability of degrading long-chain aliphatic alkanes ranging from C_20 to C_33.Octane can not supported the growth of these strains,which indicate those strains possess alkane hydroxylases differing from the monooxygenase encoded on the well-known OCT plasmid of Pseudomonas oleovorans Gpo1.M.fortuitum 514 was deficient to produce extracellular surfactant,while P.aeruginosa 1785 and P.marginata 766 were surfactant producer.High cell surface hydrophobicty was observed for each strain grown on alkanes.These studies supported the conclusion that different uptake modes were adopted by alkane degrading bacteria.