Quantitative detection of engineered nanoparticles in tissues and organs: An investigation of efficacy and linear dynamic ranges using ICP-AES |
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Authors: | H C Fischer S Fournier-Bidoz W C W Chan K S Pang |
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Institution: | (1) Department of Materials Science and Engineering, Institute of Biomaterials and Biomedical Engineering, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, M5S 3G9, Canada;(2) Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario, M5S 3M2, Canada |
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Abstract: | The absence of effective non-isotopic quantification methods to determine in vivo nanoparticle kinetics and distribution is a key obstacle to the development of various biomedical nanotechnologies. This
paper presents a novel adaptation of the established technology of Inductively Coupled Plasma-Atomic Emission Spectroscopy
(ICP-AES) to a simple technique intended to address this obstacle. Applicability to three varieties of nanoparticles, (CdSe/ZnS
quantum dots (QD), gold nanoparticles, and Fe3O4 nanoparticles) was investigated, and particle detection sensitivity was shown in moles of particles per gram of tissue. Using
gold nanoparticles, increased particle size corresponded with lower molar detection thresholds. Minimum linear detection ranges
of 2.5 orders of magnitude for QDs and 1.5 orders of magnitude for all three sizes of gold were demonstrated. The detection
of the Fe3O4 particles was hampered by the natural presence of Fe2+ in tissues, showing that the technique is not suitable for measuring nanoparticles composed of endogenous elements. These
detection levels and ranges demonstrate that this technique is useful for quantifying nanoparticles in excised organs, after
in vivo dosing. |
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Keywords: | nanoparticle in vivo quantitation quantum dot ICP-AES non-isotopic detection |
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