人肝癌细胞BEL-7402中热休克蛋白70相伴甲胎蛋白的实验研究

为研究人肝癌细胞BEL-7402中热休克蛋白70(HSP70)与甲胎蛋白(AFP)的相互作用,采用免疫化学和免疫荧光检测HSP70和AFP在肝癌细胞中的表达和定位.HSP70与AFP的相互关系通过免疫共沉淀和蛋白印迹杂交进行分析.结果免疫化学显示人肝癌细胞BEL-7402中存在高水平的HSP70和AFP共表达,均定位于细胞浆.AFP存在于HSP70单抗的免疫沉淀中,而HSP70则存在于AFP单抗的免疫沉淀中.结果表明人肝癌细胞BEL-7402中HSP70与AFP相伴.两者之间的相互关系研究将成为探讨肝癌的发生和免疫治疗的新途径.

Heat shock protein 70 chaperoned alpha-fetoprotein in Human hepatocellular carcinoma cell line BEL-7402

To investigate the interaction between heat shock protein 70(HSP70) and alpha-fetoprotein(AFP) in human hepatocellular carcinoma cell line BEL-7402,the expression and localization of HSPT0 and AFP in human hepatocellular carcinoma cell line BEL-7402 were determined by immunocytochemistry and indirected immunofluorescence cytochemicai staining.The interaction between HSP70 and AFP in hepatocarcinoma cells was analyzed by immunoprecipitation and Western Blot.Immunocytochemical staining detection showed hepatocellular cancer cell BEL-7402 expressed high level of HSP70 and AFP synchronously.Both on them were stained in cell plasma.AFP existed in the immunoprecipitate of anti-HSP70 mAb, while there was HSP70 in the immunoprecipitate of anti-AFP mAb.The results suggest that HSP70 chaperones alpha-fetoprotein in human hepatocellular carcinoma cell BEL-7402.The interaction between HSPT0 and AFP in human hepatocarcinoma cell can be a new route for studying the pathogenesis and immunotherapy of hepatocarcinoma.