2种块菌的物种分子鉴定与系统地位分析

以采自内蒙古贺兰山地区的2种块菌为实验材料,分别运用形态学和分子生物学方法进行物种鉴定,并通过与GenBank数据库信息比对,利用Mega5.1软件构建系统进化树,分析其进化关系并确定2种块菌的进化地位。结果表明:两菌株子囊果都为地下生;其中,菌株order-a为灰黄色,近球形到不规则形,较硬,表面被覆微绒毛;菌株tuber-b呈黄褐色,质地均匀,表面有疣状物,顶端呈凹陷状,全株带有干果般特殊的香气。经分子生物学方法分析认为菌株order-a为块菌属的一种,tuber-b为冬块菌(Tuber brumale),并初步确定为内蒙古新记录种。

Molecular Identification and Analysis of Phylogenetic Position for Two Truffles

Two truffles were taken as experimental materials which were from Helan mountain area in Inner Mongolia, and they were identified basing on the morphological and molecular methods. In the process of experiment, the DNA sequences were blasted using the GenBank, and phylogenetic trees were constructed by using Mega5.1 software. The results show that two strains of ascoma are hypogeous. Thereinto, order a is sallow, subsphaeroidal, to irregular shape, and with microvilli on surface. And tuber b is yellowish brown, uniform texture, with a wart on surface, and there is a special aroma of dried fruit. Through the analysis of molecular biological methods, order a was identified as a species of Tuber genus, tuber b is Tuber brumale, and the tuber b is identified as a new record species in Inner Mongolia.