| 短叶对齿藓组织培养的研究 |
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| 引用本文： | 高叶青,任冬梅,赵小丹,赵东平,隋嫣鸿.短叶对齿藓组织培养的研究[J].西北植物学报,2016,36(9):1900-1904. |
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| 作者姓名： | 高叶青 任冬梅 赵小丹 赵东平 隋嫣鸿 |
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| 作者单位： | (内蒙古大学 生命科学学院，呼和浩特010021) |
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| 基金项目： | 国家自然科学基金(31360142, 31260046)； |
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| 摘 要： | 以采自白云鄂博主矿区的短叶对齿藓为试验材料,研究了不同消毒剂以及不同浓度植物激素6-BA和IAA对短叶对齿藓愈伤组织诱导和分化的影响。结果表明:短叶对齿藓配子体最佳消毒剂及作用时间为体积浓度百分比75%乙醇浸泡30s,再用质量浓度0.1g/L升汞消毒90s;采用Knop培养基培养短叶对齿藓茎叶段,质量浓度为0.1mg/L 6-BA促进愈伤组织分化形成配子体,质量浓度1.0mg/L 6-BA则抑制短叶对齿藓愈伤组织形成,IAA有助于原丝体的萌发生长。
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| 关 键 词： | 短叶对齿藓 组织培养 植物激素 |
Tissue Culture of Didymodon tectorum |
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| GAO Yeqing,REN Dongmei,ZHAO Xiaodan,ZHAO Dongping,SUI Yanhong.Tissue Culture of Didymodon tectorum[J].Acta Botanica Boreali-Occidentalia Sinica,2016,36(9):1900-1904. |
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| Authors: | GAO Yeqing REN Dongmei ZHAO Xiaodan ZHAO Dongping SUI Yanhong |
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| Abstract: | With Didymodon tectorum collected from the main mine of Baiyun obo as experimental material, we studied the effect of different disinfectants and different concentrations of plant hormone 6 BA and IAA, respectively on callus induction and differentiation. It showed that the best disinfectant for D. tectorum gametophyte is 75% alcohol immersion for 30 s and 0.1 g/L mercuric chloride for 90 s. Using Knop medium to culture the upper stem leaf segments of D. tectorum, and a low concentration of 6 BA (0.1 mg/L) promotes callus differentiation and gametophyte formation. However, high concentration of 6 BA (1.0 mg/L) restrains the callus formation. IAA helps protonemata germinate. |
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| Keywords: | Didymodon tectorum tissue culture plant hormone |
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