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| 硫糖铝对幽门螺杆菌VacA IgY的保护作用的动物实验评价 | |
| 引用本文： | 郭丽媛,吴金英,黄伟,杨致邦,吴敏,马小京.硫糖铝对幽门螺杆菌VacA IgY的保护作用的动物实验评价[J].中国微生态学杂志,2010,22(11):988-992. |
| 作者姓名： | 郭丽媛 吴金英 黄伟 杨致邦 吴敏 马小京 |
| 作者单位： | 1. 青岛大学医学院附属烟台毓璜顶医院,山东,烟台,264000 2. 西南大学荣昌校区,动物医学系,重庆,402460 3. 重庆医科大学,基础医学实验教学中心病原生物学与免疫学实验室,神经科学研究中心,重庆,400016 |
| 基金项目： | 重庆市科委基金(CSTC,2005EA5020) |
| 摘 要： | 目的评价硫糖铝在胃内对H.pyloriVacA IgY型抗体的保护作用,为制备H.pyloriVacA IgY型抗体口服剂提供实验依据。方法大量诱导工程菌DH5α-vacA-pQE30,表达并纯化重组蛋白VacA。以VacA为抗原接种洛曼母鸡,制备纯化的IgY。建立H.pylori感染的小鼠模型,在不同浓度的IgY液中分别加入30%硫糖铝,灌胃后观察胃黏膜慢性炎症反应以评价硫糖铝在胃内对VacA IgY的保护作用。结果在小鼠胃内,0.5mg IgY+30%硫糖铝/天灌胃小鼠即可有效防治H.pylori感染引起的胃黏膜损害,与不加硫糖铝比较,其效果提高了8倍。结论 30%以上的硫糖铝在小鼠胃内可增强VacA IgY对低pH和胃蛋白酶的耐受能力,是较理想的抗H.pyloriVacA IgY型抗体保护剂。 |
| 关 键 词： | 幽门螺杆菌 细胞空泡毒素抗原 卵黄抗体 硫糖铝 保护剂 |
Evaluation on protective effects of sucralfate on H.pylori-VacA-IgY in animal experiments |
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| GUO Li-yuan,WU Jin-ying,HUANG Wei,YANG Zhi-bang,WU Min,MA Xiao-jing.Evaluation on protective effects of sucralfate on H.pylori-VacA-IgY in animal experiments[J].Chinese Journal of Microecology,2010,22(11):988-992. | |
| Authors: | GUO Li-yuan WU Jin-ying HUANG Wei YANG Zhi-bang WU Min MA Xiao-jing |
| Institution: | GUO Li-yuan1,WU Jin-ying1,HUANG Wei2,YANG Zhi-bang3,WU Min3,MA Xiao-jing3(1.The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College,Yantai 264000,China,2.Department of Zoological Medicine Rongchang Campus,Southwest University,Chongqing 402460,3.Department of Pathobiology and Immunology Experimental Teaching Center of Basic Medicine,Institute of Neuroscience,Chongqing Medical University,Chongqing 400016,China) |
| Abstract: | Objective To evaluate the protective effects of suczalfate on anti-H, pylori VacA immunoglobulin yolk （VacA IgY） intragastricalIy and provide experimental basis for preparation of oral-administration IgY. Method The recombinant vacuolate cytotoxin antigen（VacA） was expressed in engineering bacteria DHSct-vacA-pQE30 and purified. VacA IgY was prepared by inoculating purified VacA in Lohman hens and then depurated. Mice infected by H. py/or/were intragastrically administered 30% sucralfate and IgY solutions of different concentrations. The chronic inflammstory reaction of gastric mucosa was observed to evaluate the protective effects of sucralfate on VacA IgY in stomach. Result Intragastric administration with 0.5 mg IgY plus 30% sueralfate per day could effectively prevent and cure the injury of gastric mucosa caused by 1t. pylori infection. The protective effect of VacA IgY group containing 30% sucralfate was eight times as that of the groups without sucralfate. Conclusion 30% or higher concentration of sucralfate can enhance the tolerance of VacA IgY to low pH value and pepsin intragastrically and may be an ideal protectant for IgY. |
| Keywords: | Helicobacter pylori Vacuolate cytotoxin antigen Immunoglobulin yolk Sucralfate Protectant |
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