Binding of ATP to heat shock protein 90: evidence for an ATP-binding site in the C-terminal domain. |
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Authors: | Cyrille Garnier Daniel Lafitte Philipp O Tsvetkov Pascale Barbier Jocelyne Leclerc-Devin Jean-Marc Millot Claudette Briand Alexander A Makarov Maria G Catelli Vincent Peyrot |
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Affiliation: | CNRS-UPR 1524, ICGM, 24 rue du Faubourg Saint Jacques, Paris 75014, France. |
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Abstract: | The presence of a nucleotide binding site on hsp90 was very controversial until x-ray structure of the hsp90 N-terminal domain, showing a nonconventional nucleotide binding site, appeared. A recent study suggested that the hsp90 C-terminal domain also binds ATP (Marcu, M. G., Chadli, A., Bouhouche, I., Catelli, M. G., and Neckers, L. M. (2000) J. Biol. Chem. 275, 37181-37186). In this paper, the interactions of ATP with native hsp90 and its recombinant N-terminal (positions 1-221) and C-terminal (positions 446-728) domains were studied by isothermal titration calorimetry, scanning differential calorimetry, and fluorescence spectroscopy. Results clearly demonstrate that hsp90 possesses a second ATP-binding site located on the C-terminal part of the protein. The association constant between this domain of hsp90 and ATP-Mg and a comparison with the binding constant on the full-length protein are reported for the first time. Secondary structure prediction revealed motifs compatible with a Rossmann fold in the C-terminal part of hsp90. It is proposed that this potential Rossmann fold may constitute the C-terminal ATP-binding site. This work also suggests allosteric interaction between N- and C-terminal domains of hsp90. |
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