Identification of acetylcholine and propionylcholine in bull spermatozoa by integrate pyrolysis, gas chromatography and mass spectrometry |
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| Authors: | Michael R Bishop BV Rama Sastry William B Stavinoha |
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| Institution: | 1. Department of Pharmacology, Vanderbily University, Nashville, Tenn., U.S.A.;2. Department of Pharmacology, University of Texas Health Sciences Center, San Antonio, Texas, U.S.A. |
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| Abstract: | Acetylcholine was identified in fertile bull spermatozoa using combined pyrolysis gas-chromatography and mass spectrometry. Bull spermatozoa contain other minor choline esters in smaller quantities than acetylcholine. One of the minor choline esters is possibly propionycholine. The spermatozoa from infertible bulls exhibited low motility and very low levels of acetylcholine. |
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