| 激发子诱发小麦叶肉细胞原生质体微管骨架排列格局与[Ca2+]cyt的变化 |
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| 引用本文： | 刘刚,党磊,王冬梅. 激发子诱发小麦叶肉细胞原生质体微管骨架排列格局与[Ca2+]cyt的变化[J]. 分子细胞生物学报, 2007, 40(4): 205-213 |
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| 作者姓名： | 刘刚 党磊 王冬梅 |
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| 作者单位： | 河北农业大学生命科学学院，浙江大学生命科学学院，河北农业大学生命科学学院 保定 071001，杭州 310058，保定 071001 |
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| 基金项目： | 国家自然科学基金;河北省自然科学基金;国家重点实验室基金;教育部科学技术研究重点项目 |
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| 摘 要： | 以小麦叶肉细胞原生质体-激发子互作为研究体系,通过免疫荧光标记和Ca~(2 )荧光染料的装载并结合药理学试验,借助激光共聚焦扫描显微镜观察,探讨小麦抵抗叶锈菌侵染过程中微管骨架和Ca~(2 )之间的内在联系。试验结果表明,激发子处理可引起抗性品种原生质体[Ca~(2 )]_(cyt)的升高并诱发微管骨架的解聚,预解聚微管骨架,再用激发子处理,可使抗性品种原生质体[Ca~(2 )]_(cyt)的升高幅度增加。
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| 关 键 词： | 微管 激发子 原生质体 小麦 |
| 修稿时间： | 2007-01-042007-05-10 |
THE INFLUENCE OF ELICITOR ON THE DISTRIBUTION PATTERN OF MICROTUBULE AND THE CYTOSOLIC CALCIUM IN MESOPHYLL PROTOPLAST OF WHEAT |
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| LIU Gang,DANG Lei,WANG Dong Mei. THE INFLUENCE OF ELICITOR ON THE DISTRIBUTION PATTERN OF MICROTUBULE AND THE CYTOSOLIC CALCIUM IN MESOPHYLL PROTOPLAST OF WHEAT[J]. Journal of Molecular Cell Biology, 2007, 40(4): 205-213 |
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| Authors: | LIU Gang DANG Lei WANG Dong Mei |
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| Affiliation: | 1.College of Life Science, Agricultural University of Hebei, Baoding 071001; 2.College of Life Science, University of Zhejiang, Hangzhou 310058 |
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| Abstract: | By using immunofluorescence labeling technique and incubating the Fluo-3 AM of Ca~(2 ) fluorescein,wheat(Triticum aestivum) cultivars Lovrin 10 and 5389 were selected as mesophyll pro- toplast material interaction with elicitor in this paper for studying the relationship between the pattern of microtubule and [Ca~(2 )]_(cyt).Confocal Laser Scanning Microscopy(CLSM) was used and pharmacologic examination was set.The results indicated:when treated with elicitor,the cytosolic calcium in the protoplasts from the Lovrin 10 was increasing and the pattern of microtubule showed depolymerization. In the protoplasts which MTs were depolymerized by oryzalin prior to elicitor treatment,the increase of cytosolic calcium was even higher than that in the one which was not depolymerized. |
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| Keywords: | Ca2 |
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