Specification of amino acid residues essential for the catalytic reaction of cold-active protein-tyrosine phosphatase of a psychrophile, Shewanella sp |
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Authors: | Tsuruta Hiroki Tamura Jun Yamagata Hiroshi Aizono Yasuo |
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Institution: | Center for Cooperative Research and Development, Kobe University, Japan. tsuruta@ans.kobe-u.ac.jp |
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Abstract: | Protein-tyrosine phosphatase EC 3.1.3.48] from a psychrophile, Shewanella sp. shows high activity at low temperatures and has the conserved amino acid sequence of protein-Ser/Thr-phosphatases. Site-directed mutagenesis with the conserved amino acid residues indicated that His148 could be important as a general acid catalyst and Asp115 assists the protonation with His148 of the leaving group of a substrate, and that Asp76 and Asp112 were involved in binding to magnesium ions. |
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