Streptomyces sp.FIM-16-06产他克莫司摇瓶发酵条件的优化

采用单因素试验和正交试验研究发酵培养基组成,优化Streptomyces sp. FIM-16-06产他克莫司的发酵条件,探讨摇瓶发酵的主要影响因子初始p H、装液量、转速等发酵参数的影响。确定了适宜的发酵培养基和发酵参数:6. 0%玉米淀粉、2. 0%黄豆粉、2. 0%葡萄糖和0. 5%玉米浆,初始pH 7. 5,装液量50 m L/500 m L三角瓶,种子菌龄48 h,接种量10%,摇床转速250 r/min,发酵温度27℃,发酵周期7 d。优化后的发酵水平较原生产工艺提高60%以上。他克莫司的优化发酵工艺为其工业化生产奠定了基础。

Optimization of fermentation conditions of tacrolimus-producing strain Streptomyces sp. FIM-16-06

In order to optimize the fermentation conditions for tacrolimus-producing strain Streptomyces sp.FIM-16-06,the single factor experiments and orthogonal experiments were adopted to investigate the liquid fermentation medium and culturing parameters of Streptomyces sp.FIM-16-06.The results revealed that the appropriate fermentation medium composition and optimal fermentation parameters were determined as follows:fermentation medium containing corn starch 6.0%,glucose 2.0%,soybean flour 2.0%and corn steep liquor 0.5%,while the initial pH 7.5,filling volume 50 mL/500 mL shake flask,cultivation time of seed 48 h,inoculum size 10%,shaker’s rotating speed 250 r/min,fermentation temperature 27℃and fermentation time 7 d.The tacrolimus yield of Streptomyces sp.FIM-16-06 under the optimized fermentation conditions was 60%higher than that of the original.The optimal fermentation conditions of Streptomyces sp.FIM-16-06 laid the foundation for further industrial fermentation.