Contrasting colonization and plant growth promoting capacity between wild type and a gfp-derative of the endophyte Pseudomonas putida W619 in hybrid poplar |
| |
Authors: | Nele Weyens Jana Boulet Dirk Adriaensen Jean-Pierre Timmermans Els Prinsen Sandra Van Oevelen Jan D’Haen Karen Smeets Daniel van der Lelie Safiyh Taghavi Jaco Vangronsveld |
| |
Institution: | 1. Environmental Biology, Hasselt University, Agoralaan Building D, B-3590, Diepenbeek, Belgium 2. Laboratory of Cell Biology & Histology, Department of Veterinary Sciences, Antwerp University, Groenenborgerlaan 171, 2020, Antwerp, Belgium 3. Department of Biology, Groenenborgercampus, Antwerp University, Groenenborgerlaan 171, 2020, Antwerp, Belgium 4. Institute for Materials Research, Hasselt University, Wetenschapspark 1, B-3590, Diepenbeek, Belgium 5. Biodiversity, Phylogeny & Population Studies, Hasselt University, Agoralaan Building D, B-3590, Diepenbeek, Belgium 6. Research Triangle Institute (RTI), International 3040 Cornwallis Road, Research Triangle Park, NC, 27709-2194, USA 7. Brookhaven National Laboratory (BNL), Biology Department, Building 463, Upton, NY, 11973, USA
|
| |
Abstract: | This study aims to investigate the colonization of poplar by the endophyte Pseudomonas putida W619 and its capacity to promote plant growth. Poplar cuttings were inoculated with P. putida W619 (wild-type or gfp-labelled). The colonization of both strains was investigated and morphological, physiological and biochemical parameters were analyzed to evaluate plant growth promotion. Inoculation with P. putida W619 (wild-type) resulted in remarkable growth promotion, decreased activities of antioxidative defence related enzymes, and reduced stomatal resistance, all indicative of improved plant health and growth in comparison with the non-inoculated cuttings. In contrast, inoculation with gfp-labelled P. putida W619 did not promote growth; it even had a negative effect on plant health and growth. Furthermore, compared to the wildtype strain, colonization by the gfp-labelled P. putida W619::gfp1 was much lower; it only colonized the rhizosphere and root cortex while the wild-type strain also colonized the root xylem vessels. Despite the strong plant growth promoting capacity of P. putida W619 (wild-type), after gfp labelling its growth promoting characteristics disappeared and its colonization capacity was strongly influenced; for these reasons gfp labelling should be applied with sufficient caution. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|