Biosynthesis and metabolic engineering of glucosinolates |
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| Authors: | Mikkelsen M D Petersen B L Olsen C E Halkier B A |
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| Institution: | (1) Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University,, DK;(2) Center for Molecular Plant Physiology (PlaCe), The Royal Veterinary and Agricultural University, and, DK;(3) Department of Chemistry, The Royal Veterinary and Agricultural University, Frederiksberg C, Copenhagen, Denmark, DK |
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| Abstract: | Summary. Glucosinolates are amino acid-derived natural plant products found throughout the Capparales order. Glucosinolates and their
degradation products have a wide range of biological activities, e.g. in plant defense as deterrents against insect and fungi. The conversion of amino acids to aldoximes is a key step in glucosinolate
biosynthesis. This step is catalyzed by cytochromes P450 from the CYP79 family. The post-aldoxime enzymes in the glucosinolate
pathway have high substrate-specificity for the functional group and low substrate-specificity for the side chain. Therefore,
we have been able to metabolically engineer new glucosinolate profiles into Arabidopsis by altering the levels of endogenous
CYP79s and by introducing exogenous CYP79s. The approach has great potential for design of metabolically engineered plants
with improved pest resistance and increased nutritional value.
Received December 7, 2001 Accepted December 21, 2001 |
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| Keywords: | : Glucosinolates Metabolic engineering CYP79s Aldoximes Arabidopsis CYP83s |
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