Identification of the KDR tyrosine kinase as a receptor for vascular endothelial cell growth factor. |
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Authors: | B I Terman M Dougher-Vermazen M E Carrion D Dimitrov D C Armellino D Gospodarowicz P B?hlen |
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Institution: | Medical Research Division, Lederle Laboratories, Pearl River, New York 10965. |
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Abstract: | Vascular endothelial cell growth factor (VEGF), also known as vascular permeability factor, is an endothelial cell mitogen which stimulates angiogenesis. Here we report that a previously identified receptor tyrosine kinase gene, KDR, encodes a receptor for VEGF. Expression of KDR in CMT-3 (cells which do not contain receptors for VEGF) allows for saturable 125I-VEGF binding with high affinity (KD = 75 pM). Affinity cross-linking of 125I-VEGF to KDR-transfected CMT-3 cells results in specific labeling of two proteins of M(r) = 195 and 235 kDa. The KDR receptor tyrosine kinase shares structural similarities with a recently reported receptor for VEGF, flt, in a manner reminiscent of the similarities between the alpha and beta forms of the PDGF receptors. |
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