利用分子标记定位水稻野败型核质互作雄性不育恢复基因

以籼稻恢复系圭６３０与粳型广亲和品种０２４２８的Ｆ１代花药培养，获得８１个双单倍体（ＤＨ），构建了有２３３个ＲＦＬＰ标记的分子图谱。用籼稻野败型不育系珍汕９７Ａ测定各ＤＨ系的恢复性，并将恢复性作为数量性状进行ＱＴＬ的区间作图分析，鉴别出８个基因座位，其中有２个基因座位，Ｒｆｉ－３和尾Ｒｆｉ－４，单个ＱＴＬ的基因贡献值分别是４９．６％和３５．４％，对育性恢复起主要作用，定为主效基因座位，位于第三和四染色体上，其它６个基因座位对育性恢复亦有一定的影响。表明野败型雄性不育恢复性是受主效基因和微效基因共同控制的性状。

RFLP Mapping of Genes Confering Fertility Restoration of Wild Cytoplasmic- genetic Male Sterility in Rice (Oryza sativa subsp, indica)

An RFLP linkage map including 233 loci was constructed based on a doubled haploid(DH)population derived from anther culture of a cross between an indica restorer line Gui 630 and a japonica wide compatibility variety 02428. The restoring ability of DH lines was determined by their respective test crosses with Zhenshan 97A, a wild cytoplasmic male sterile line. Interval mapping approach was used to detect the quantitative trait loci associated with fertility restoration,and 8 QTLs were identified. Among them two major genes, Rfi-3 and Rfi-4,which were responsible for 49.6% and 35.4%of the phenotypic variation were located on chromosome 3 and 4 respectively. The other 6 QTLs affected the performance of restoration to some extent.The results indicate that the fertility restoration of WA type sterility is genetically controlled by the major genes,to gether with some minor genes.