Strain typing of Lentinula edodes by random amplified polymorphic DNA assay |
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| Authors: | Yinfang Zhang Francis I Molina |
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| Institution: | Mycology and Botany Department, American Type Culture Collection, 12301 Parklawn Drive, Rockville, MD 20852-1776, USA |
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| Abstract: | Abstract Single 10-base primers were used to generate randomly amplified polymorphic DNA (RAPD) markers in the shiitake mushroom, Lentinula edodes . Seven primers produced polymorphisms in all 15 strains tested, producing 12–19 bands ranging from 0.34 to 2.52 kb. Thirteen of the 15 strains had unique DNA fingerprints, whereas L. edodes ATCC 28759 and ATCC 28760 exhibited identical RAPD profiles for all the primers. Molecular-genetic markers obtained with the RAPD assay can be used to differentiate strains of L. edodes and have potential applications in mushroom breeding and strain improvement programs. |
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| Keywords: | Lentinula edodes Strain typing Random amplified polymorphic DNA |
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