Type IIE and IIF Restriction Endonucleases Interacting with Two Recognition Sites on DNA |
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| Authors: | Kirsanova O V Baskunov V B Gromova E S |
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| Institution: | (1) Faculty of Chemistry, Moscow State University, Moscow, 119992, Russia;(2) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 117984, Russia |
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| Abstract: | Recent studies have shown that restriction endonucleases (REs), which are broadly used in genetic engineering and molecular biology, vary not only in nucleotide sequence of the recognition site, but also in the mechanism of their interaction with DNA. This review focuses on type IIF and IIE REs, which require simultaneous interaction with two nucleotide sequences for efficient DNA cleavage. Crystal structures of these REs and their complexes with DNA, stepwise interactions with DNA, catalytic mechanisms of DNA hydrolysis, and DNA looping are considered. Type IIE REs have provided an example of a new type of DNA–protein recognition: two copies of one recognition sequence interact specifically with two different amino acid sequences and two different structural motifs of one polypeptide chain. |
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| Keywords: | restriction endonucleases two-site mechanism structure DNA recognition kinetic schemes catalytic motifs architecture of the active center |
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