Interactions of growth inhibitory factor with hydroxyl and superoxide radicals |
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Authors: | Yanbo Shi Wenfeng Wang Jingjie Mo Liang Du Side Yao Wenxia Tang |
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Affiliation: | (1) State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, Nanjing University, Nanjing, 210093, P.R. China;(2) Laboratory of Radiation Chemistry, Shanghai Institute of Nuclear Research, Academia Sinica, Shanghai, 201800, P.R. China |
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Abstract: | To show the effects of growth inhibitory factor (Cu4Zn3MT-III) involved in the scavenging of reactive oxygen species (ROS), a pulse radiolytic study was employed using N2O-saturated Cu4Zn3MT-III aqueous solutions. It was demonstrated that the oxidizing OH radical efficiently reacted with Cu4Zn3MT-III by forming a thiyl radical RS with a second-order constant of 1.46×1011 mol l–1s–1, which was determined by competition kinetics against KSCN. The thiyl radical RS reacted rapidly and reversibly with a thiolate in Cu4Zn3MT-III to form radical anion RSSR – with a constant of 1.65×109 mol lL–1s–1 per thiolate, while the constant of the decay of this radical anion was 2.72×105 s–1, and the equilibrium constant of the formation for RSSR – was 6.08×103 mol–1 l. These values were close to those of Cd5Zn2MT-II. The SOD activity of Cu4Zn3MT-III to quench O2 –was assayed by the riboflavine-methionine-nitrobluetetrazolium (NBT) method which catalyzed the dismutation of superoxide (O2 –) at pH 7.8 with an IC50 value of 1.50×10–6 M for Cu4Zn3MT-III and 1.62×10–6 M for Cd5Zn2MT-II. Additionally, the down-regulation of GIF may be a main factor in the decrease of the scavenging ability for the free OH and O2 –radicals, which is possibly associated with the pathogenesis of neurodegenerative disease. |
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Keywords: | Alzheimer's disease (AD) Cd5Zn2MT-II superoxydral radical (O2 /content/k102455m2451v545/xxlarge8901.gif" alt=" sdot" align=" BASELINE" BORDER=" 0" > – ) growth inhibitory factor (GIF or Cu4Zn3MT-III) hydroxyl radical (OH /content/k102455m2451v545/xxlarge8901.gif" alt=" sdot" align=" BASELINE" BORDER=" 0" >) |
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