Cloning of Klebsiella pneumoniae pqq genes and PQQ biosynthesis in Escherichia coli |
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| Authors: | J.J.M. Meulenberg E. Sellink W.A.M. Loenen N.H. Riegman M. Van Kleef P.W. Postma |
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| Affiliation: | E.C. Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands. |
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| Abstract: | We have cloned genes from Klebsiella pneumoniae which are required for pyrroloquinoline quinone (PQQ) biosynthesis. The cloned 6.7 kb fragment can complement several chromosomal pqq mutants. Escherichia coli strains are unable to synthesize PQQ but E. coli strains containing the cloned 6.7 kb K. pneumoniae fragment can synthesize PQQ in large amounts and E. coli pts mutants can be complemented on minimal glucose medium by this clone. |
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| Keywords: | Pyrroloquinoline quinone Klebsiella pneumoniae Cloning pqq Gene Glucose dehydrogenase |
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