| 姜荷花炭疽病病原菌分离与鉴定 |
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| 引用本文： | 赵健,赵志国,李秀娟,张翠萍,区蝉,林萌,仇硕. 姜荷花炭疽病病原菌分离与鉴定[J]. 广西植物, 2011, 31(4): 531-535. DOI: 10.3969/j.issn.1000-3142.2011.04.021 |
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| 作者姓名： | 赵健 赵志国 李秀娟 张翠萍 区蝉 林萌 仇硕 |
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| 作者单位： | 1. 广西壮族自治区中国科学院广西植物研究所,广西桂林,541006
2. 桂林市石山绿化试验站,广西桂林,541001 |
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| 基金项目： | 广西自然科学基金(桂科自0991225); 广西植物研究所基本科研业务费项目(桂植业09018,桂植业09008)~~ |
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| 摘 要： | 利用常规组织分离法对姜荷花炭疽病病原菌进行了分离,并对分离菌株进行培养、纯化、回接和重新分离,最后利用形态学和分子生物学技术对致病菌株进行了鉴定.结果表明:从感病叶片中分离得到6株病原菌,病原菌室外回接发现只有菌株Cum-3致病,Cum-3室外回接致病率96.7％,室内回接致病率100％.形态学鉴定,Cum-3为镰孢酶...
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| 关 键 词： | 姜荷花 炭疽病 分离 鉴定 |
Isolation and identification of pathogens that caused Curcuma alismatifolia anthracnose |
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| ZHAO Jian,ZHAO Zhi-Guo,LI Xiu-Juan,ZHANG Cui-Ping,OU Chan,LIN Meng,QIU Shuo. Isolation and identification of pathogens that caused Curcuma alismatifolia anthracnose[J]. Guihaia, 2011, 31(4): 531-535. DOI: 10.3969/j.issn.1000-3142.2011.04.021 |
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| Authors: | ZHAO Jian ZHAO Zhi-Guo LI Xiu-Juan ZHANG Cui-Ping OU Chan LIN Meng QIU Shuo |
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| Affiliation: | ZHAO Jian1,ZHAO Zhi-Guo1,LI Xiu-Juan1,ZHANG Cui-Ping1,OU Chan1,LIN Meng2,QIU Shuo1(1.Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences,Guilin 541006,China,2.Limestone Greening Station of Guilin,Guilin 541001,China) |
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| Abstract: | The pathogens that caused Curcuma alismatifolia anthracnose were isolated using usual tissue isolation.Strains were cultivated,purified,reinoculated in the field and laboratory.The strain that caused the anthracnose was re-isolated.Morphological and molecular biological techniques were used to identify the strain.Results showed that 6 strains were isolated from infected leaves of C.alismatifolia,but only Cum-3 caused the anthracnose.The incidences of Cum-3 disease reinoculated in the field and laboratory we... |
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| Keywords: | Curcuma alismatifolia anthracnose isolation identification |
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