| 珍稀濒危植物银鹊树体细胞胚时期同工酶分析 |
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| 引用本文： | 张博,景丹龙,李晓玲,王玉兵,张德春.珍稀濒危植物银鹊树体细胞胚时期同工酶分析[J].广西植物,2011,31(4):526-530. |
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| 作者姓名： | 张博 景丹龙 李晓玲 王玉兵 张德春 |
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| 作者单位： | 三峡大学生物技术研究中心,湖北宜昌,443002 |
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| 基金项目： | 国家自然科学基金(30670202); 湖北省天然产物开发与利用重点实验室开放基金(2008DP09)~~ |
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| 摘 要： | 采用聚丙烯酰胺凝胶电泳技术对银鹊树体细胞胚胎发生过程中的酯酶(EST)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和淀粉酶(AMY)进行了同工酶分析.结果表明:球形胚时期的EST、POD、SOD、AMY同工酶活性最强；在体细胞胚胎形态建成过程中,SOD同工酶有新酶的合成,而POD同工酶则表现为活性表达增加并有新酶合...
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| 关 键 词： | 银鹊树 体细胞胚 聚丙烯酰胺凝胶电泳 同工酶 酶带 |
Studies on isozymes during somatic embryogenesis of Tapiscia sinensis |
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| ZHANG Bo,JING Dan-Long,LI Xiao-Ling,WANG Yu-Bing,ZHANG De-Chun.Studies on isozymes during somatic embryogenesis of Tapiscia sinensis[J].Guihaia,2011,31(4):526-530. |
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| Authors: | ZHANG Bo JING Dan-Long LI Xiao-Ling WANG Yu-Bing ZHANG De-Chun |
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| Institution: | ZHANG Bo,JING Dan-Long,LI Xiao-Ling,WANG Yu-Bing,ZHANG De-Chun(Biotechnology Research Center and Key Laboratory of Plant Natural Products of Hubei,China Three Gorges University,Yichang 443002,China) |
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| Abstract: | Polyacrylamide gel electrophoresis was used as a tool to analyze isozyme patterns such as peroxidase(POD),esterase(EST),amylase(AMY)and superoxide dismutase(SOD)in somatic embryogenesis of Tapiscia sinensis.The results showed that a higher expression of EST,POD,AMY and SOD isoenzymes indicated that there was a higher physiological metabolic rate in the stage of globular embryos,and SOD became more which might be associated with the processes of somatic cells from the differentiation into embryonic regenerat... |
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| Keywords: | Tapiscia sinensis somatic embroyos Polyacrylamide gel electrophoresis isoenzyme enzyme bands |
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