The F1FO ATP synthase genes in Methanosarcina acetivorans are dispensable for growth and ATP synthesis |
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Authors: | Regina Saum,Katharina Schlegel,Benjamin Meyer,& Volker Mü ller |
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Affiliation: | Molecular Microbiology &Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe-University Frankfurt, Frankfurt am Main, Germany |
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Abstract: | There is a long-standing discussion in the literature, based on biochemical and genomic data, whether some archaeal species may have two structurally and functionally distinct ATP synthases in one cell: the archaeal A1AO together with the bacterial F1FO ATP synthase. To address a potential role of the bacterial F1FO ATP synthase, we have exchanged the F1FO ATPase gene cluster in Methanosarcina acetivorans against a puromycin resistance cassette. Interestingly, the mutant was able to grow with no difference in growth kinetics to the wild type, and cellular ATP contents were identical in the wild type and the mutant. These data demonstrate that the F1FO ATP synthase is dispensable for the growth of M. acetivorans . |
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Keywords: | archaea methanogens ATP synthase F1FO A1AO |
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