| 化学修饰寡核苷酸在核酸配基扩增技术中的应用 |
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| 引用本文： | 张兴梅,孙曼霁.化学修饰寡核苷酸在核酸配基扩增技术中的应用[J].生命科学,2002,14(4):238-241. |
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| 作者姓名： | 张兴梅 孙曼霁 |
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| 作者单位： | 军事医学科学院毒物药物研究所,北京,100850 |
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| 摘 要： | 核酸酸基扩增技术（SELEX)可从极大容量的随机寡核苷酸文库中筛选得到与靶分子高特异性和高亲和力结合的核酸配基。对寡核苷酸进行化学修饰，可以提高核酸配基的稳定性，增加其功能多样性及生物利用度。SELEX在基础研究、诊断和治疗试剂的研制及药物筛选等领域有广泛用途。
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| 关 键 词： | 核酸配基扩增技术 核酸配基 化学修饰 寡核苷酸 |
| 文章编号： | 1004-0374(2002)04-0238-04 |
| 修稿时间： | 2001年11月29 |
Application of chemically modified oligonucleotides in systematic evolution of ligands by exponential enrichment |
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| ZHANG Xing-Mei,SUN Man-Ji.Application of chemically modified oligonucleotides in systematic evolution of ligands by exponential enrichment[J].Chinese Bulletin of Life Sciences,2002,14(4):238-241. |
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| Authors: | ZHANG Xing-Mei SUN Man-Ji |
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| Abstract: | SELEX(systematic evolution of ligands by exponential enrichment) is a method developed to search among large random-sequence oligonucleotide library for aptamer with high-affinity and high-specific binding to defined molecular target. Modifying oligonucleotide can increase their stability, enhance their versatility and the fraction of bioavailability. SELEX will be broadlly applied in basic research and diagnostic and therapy agent and drug development. |
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| Keywords: | SELEX aptamer chemically modifying oligonucleotides |
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