Solubilization of the receptor for N-1-naphthylphthalamic Acid |
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Authors: | Sussman M R Gardner G |
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Affiliation: | Shell Development Company, Biological Sciences Research Center, P.O. Box 4248, Modesto, California 95352. |
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Abstract: | A receptor protein for the auxin transport inhibitor, N-1-naphthylphthalamic acid (NPA), has been solubilized from corn coleoptile membranes using Triton X-100. [3H]NPA binding activity of the receptor was compared in soluble and membrane-bound states. Both activities are abolished by treatment with trypsin. Differences between the two are observed in pH optima and rates of heat inactivation. |
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