Potato Expressed Sequence Tag Generation and Analysis using Standard and Unique cDNA Libraries |
| |
Authors: | Barry Flinn Charlotte Rothwell Rebecca Griffiths Martin Lägue David DeKoeyer Ravinder Sardana Patrice Audy Claudia Goyer Xiu-Qing Li Gefu Wang-Pruski Sharon Regan |
| |
Institution: | (1) Canadian Potato Genome Project, BioAtlantech Unit, 921 College Hill Road, Fredericton, New Brunswick, E3B 6Z9, Canada;(2) Potato Research Centre, Agriculture and Agri-Food Canada, 850 Lincoln Rd, P.O. Box 20280, Fredericton, New Brunswick, E3B 4Z7, Canada;(3) Department of Plant and Animal Sciences, Cox Institute, Nova Scotia Agricultural College, Box 550, Truro, Nova Scotia, B2N 5E3, Canada;(4) Department of Biology , Queen’s University, Kingston, Ontario, K7L 3N6, Canada |
| |
Abstract: | To help develop an understanding of the genes that govern the developmental characteristics of the potato (Solanum tuberosum), as well as the genes associated with responses to specified pathogens and storage conditions, The Canadian Potato Genome
Project (CPGP) carried out 5′ end sequencing of regular, normalized and full-length cDNA libraries of the Shepody potato cultivar,
generating over 66,600 expressed sequence tags (ESTs). Libraries sequenced represented tuber developmental stages, pathogen-challenged
tubers, as well as leaf, floral developmental stages, suspension cultured cells and roots. All libraries analysed to date
have contributed unique sequences, with the normalized libraries high on the list. In addition, a low molecular weight library
has enhanced the 3′ ends of our sequence assemblies. Using the combined assembly dataset, unique tuber developmental, cold
storage and pathogen-challenged sequences have been identified. A comparison of the ESTs specific to the pathogen-challenged
tuber and foliar libraries revealed minimal overlap between these libraries. Mixed assemblies using over 189,000 potato EST
sequences from CPGP and The Institute for Genomics Research (TIGR) has revealed common sequences, as well as CPGP- and TIGR-unique
sequences.
Electronic Supplementary Material Electronic Supplementary material is available for this article at
and accessible for authorised users. |
| |
Keywords: | common scab EST genomics late blight potato tuber |
本文献已被 PubMed SpringerLink 等数据库收录! |
|