Direct spectrophotometric analysis of hemoglobin in isoelectric focusing tube gels |
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| Authors: | R S Magliozzo C M Park J Peisach |
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| Affiliation: | 1. Department of Molecular Pharmacology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461 USA;2. Department of Molecular Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461 USA;3. Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032 USA |
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| Abstract: | Methods are described for the direct spectrophotometric analysis of human oxyhemoglobin, deoxyhemoglobin, and methemoglobin focused in polyacrylamide tube gels. Visible absorption spectra (350-650 nm) of electrofocused hemoglobin bands were recorded using a diode array rapid-scan spectrophotometer. Direct optical sampling of gels allowed the identification of focused hemoglobin valency hybrids which contain two oxidized monomers per tetramer. |
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| Keywords: | spectrophotometry hemoglobin isoelectric focusing optical spectroscopy |
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