Effect of catholyte in culture media on cell growth at extreme temperatures]

The growth of Escherichia coli cells in media whose properties were changed by the action of direct current in the cathode compartment of a diaphragm electrolyzer was studied. For this purpose, the growth medium was preliminarily treated using different method of medium preparation under different treatment conditions. The biological activity of medium was estimated by measuring the effect of these solutions on cell growth. Cells were cultivated under extremal temperature conditions, 20 degrees C and 42 degrees C (optimal temperature 37 degrees C). It was shown that at low temperatures, the cell growth considerably increases as compared to control. At elevated temperatures, the acceleration of cell death occurred, whereas in the control the deceleration of cell growth and partial cell death were observed.