Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts. |
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| Authors: | A D Bershadsky V I Gelfand T M Svitkina I S Tint |
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| Affiliation: | 1. Department of Pediatric Surgery, Seoul National University College of Medicine, Seoul, Republic of Korea;2. Department of Pediatric Surgery, Korea University College of Medicine, Seoul, Republic of Korea;3. Department of Surgery, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam, Republic of Korea;1. Department of Chemistry, Annamalai University, Chidambaram 608002, India;2. Department of Chemistry, Indian Institute of Technology Madras, Chennai 600 036, India |
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| Abstract: | Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 degrees C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 degrees C. |
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