Differential responsiveness of late passage C-6 glial cells and advanced passages of astrocytes derived from aged mouse cerebral hemispheres to cytokines and growth factor: Glutamine synthetase activity |
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Authors: | Theodosia Kazazoglou Eleni Fleischer-Lambropoulos Taxiarchis Geladopoulos Susan Kentroti Costas Stefanis Antonia Vernadakis |
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Affiliation: | (1) Eginition Hospital, University Mental Health Research Institute, 74 Vas Sophias Ave., 11528 Athens, Greece;(2) Present address: University of Colorado Health Sciences Center, 80262 Denver, CO;(3) Present address: University of Colorado Health Sciences Center, 80262 Denver, CO |
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Abstract: | In this study, we were interested to compare the responsiveness to growth factors, NGF, b-FGF and EGF and cytokines, IL1β, and TNF-α, in late passages (74–79) C6 glial cells committed astrocytes and astrocytes of advanced passages (26–28) in cultures derived from aged mouse cerebral hemispheres (MACH). Cultures were grown in either DMEM or chemically defined medium (CDM/TIPS) in order to test the effects of growth factors or cytokines. The activity of glutamine synthetase (GS), a marker for astrocytes, was used as a test parameter. We found that treatment with growth factors increased GS activity in both glial cell culture systems with the exception of EGF in C-6 glial cells. Treatment with cytokines markedly decreased GS activity in the late passage C6 glial cells whereas only TNF-α had a similar effect on MACH astrocytes. In view of the generally opposite effects of growth factors and cytokines on GS activity, we-speculate that these molecules which are also endogenously present in glial cells may play a role in the maintenance of cellular homeostasis. |
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Keywords: | C-6 glia glia derived from aged mouse brain glutamine synthetase interleukin 1β tumor necrosis factor-α NGF b-FGF EGF anti-NGF anti-bFGF anti-EGF |
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