Identification of monoclonal antibody defined epitopes on human leukocyte antigens utilizing phage display peptide libraries |
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Authors: | Qinwei Zhou Smruti A Desai Xinhui Wang Elvyra J Noronha Mariangela Neri and Soldano Ferrone |
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Institution: | (1) Imclone Systems Inc., New York, NY, 10014, U.S.A;(2) Department of Microbiology and Immunology, New York Medical College, Valhalla, NY, 10595, U.S.A |
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Abstract: | Utilizing phage display peptide libraries, we have identified and mapped the antigenic determinants recognized by mouse monoclonal antibodies (mAb) on two sets of immunologically important molecules, HLA class I and class II antigens. Anti-HLA class I mAb TP25.99 recognizes a conformational and a linear determinant on distinct regions of the HLA class I 3 domain. Anti-HLA class I mAb HO-4 recognizes a conformational determinant on the 2 domain of HLA-A2 and A28 allospecificities. Anti-HLA-DR1, -DR4, -DR6, -DR8, -DR9 mAb SM/549 recognizes a conformational determinant on the chain of HLA class II antigens. These results indicate the versatility of phage display peptide libraries to characterize antigenic determinants recognized by anti-HLA mAb. |
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Keywords: | antigenic determinants combinatorial libraries major histocompatibility antigens |
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