Genomic sequence analysis of the 238-kb swine segment with a cluster of TRIM and olfactory receptor genes located, but with no class I genes, at the distal end of the SLA class I region |
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Authors: | Asako Ando Atsuko Shigenari Jerzy K Kulski Christine Renard Patrick Chardon Takashi Shiina Hidetoshi Inoko |
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Institution: | (1) Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan;(2) Centre for Bioinformatics and Biological Computing, School of Information Technology, Division of Arts, Murdoch University, 6150 Murdoch, Western Australia, Australia;(3) Laboratoire de Radiobiologie et d’Etude du Genome, INRA-CEA, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France |
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Abstract: | Continuous genomic sequence has been previously determined for the swine leukocyte antigen (SLA) class I region from the TNF gene cluster at the border between the major histocompatibility complex (MHC) class III and class I regions to the UBD gene at the telomeric end of the classical class I gene cluster (SLA-1 to SLA-5, SLA-9, SLA-11). To complete the genomic sequence of the entire SLA class I genomic region, we have analyzed the genomic sequences of two
BAC clones carrying a continuous 237,633-bp-long segment spanning from the TRIM15 gene to the UBD gene located on the telomeric side of the classical SLA class I gene cluster. Fifteen non-class I genes, including the zinc
finger and the tripartite motif (TRIM) ring-finger-related family genes and olfactory receptor genes, were identified in the
238-kilobase (kb) segment, and their location in the segment was similar to their apparent human homologs. In contrast, a
human segment (alpha block) spanning about 375 kb from the gene ETF1P1 and from the HLA-J to HLA-F genes was absent from the 238-kb swine segment. We conclude that the gene organization of the MHC non-class I genes located
in the telomeric side of the classical SLA class I gene cluster is remarkably similar between the swine and the human segments,
although the swine lacks a 375-kb segment corresponding to the human alpha block.
The nucleotide sequence data reported in this paper have been submitted to DDBJ, EMBL, and GenBank databases under accession
numbers AB158486 and AB158487 |
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Keywords: | MHC class I region Sequencing analysis Swine Olfactory receptor Non-class I genes Ring-finger-related family |
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