A microcalorimetric study of collagen hydration in the temperature range 20–100°C by measuring the enthalpy of water evaporation from sample |
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| Authors: | I Mesropyan T V Burdzhanadze Sh G Barbakadze N G Esipova J Monaselidze |
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| Institution: | (1) Andronikashvili Institute of Physics, Academy of Sciences of Georgia, ul. Tamarashvili 6, Tbilisi, 380077, Georgia;(2) Natishvili Institute of Experimental Morphology, Academy of Sciences of Georgia, ul. Tamarashvili 6, Tbilisi, 380077, Georgia;(3) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 119991, Russia |
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| Abstract: | A new definition of the term “biopolymer hydration,” based on thermodynamic characteristics of water evaporation from biological preparations, is proposed. A new method for investigation of bound water, based on precise measurement of the enthalpy of water evaporation from the sample, using differential scanning micro-calorimetry, is developed. Adequacy of the new approach for estimating the water state in biopolymers and at various levels of structural organization of biosystems is demonstrated by study of collagen fibers as an example. |
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| Keywords: | differential scanning calorimetry determination of bound water |
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