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Molecular insights into the taxonomy of Hypanis (Bivalvia, Cardiidae, Lymnocardiinae) in the Black Sea lagoons
Authors:Luis Popa  Oana Popa  Elena Iorgu  Beatrice Kelemen  Dumitru Murariu
Affiliation:1. Grigore Antipa National Museum of Natural History, Sos. Kiseleff Nr. 1, 011341, Bucharest, Romania
2. Babes-Bolyai University, Molecular Biology Center, 42 Treboniu Laurian Street, 400271, Cluj-Napoca, Romania
Abstract:In this study, we used data from morphology and three DNA markers to assess the taxonomic status of the putative bivalve species Hypanis colorata and Hypanis angusticostata in a Black Sea lagoon, the Razelm Lake in Romania. The morphological data (the shape of shell ribs and the multivariate analysis of morphometric variance of three variables constructed as the ratios between the main dimensions of the shell) confirmed that the two analyzed species are distinct morphological entities. Three molecular markers, one from the nuclear genome (18S rRNA) and two from the mitochondrial genome (16S rRNA and COI), showed extremely reduced sequence divergence (0?C0.1%) between the two putative species. Based on these results, we suggest that H. angusticostata and H. colorata are morphotypes of a single species.
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