Sodium-dependent succinate decarboxylation by a new anaerobic bacterium belonging to the genus Peptostreptococcus |
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Authors: | Peter H Janssen Werner Liesack Claudia Kluge Sabine Seeliger Bernhard Schink Chris G Harfoot |
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Institution: | (1) Department of Biological Sciences, University of Waikato, Hamilton, New Zealand;(2) Fakultät für Biologie, Universität Konstanz, Konstanz, Germany;(3) Max-Planck-Institut für Terrestrische Mikrobiologie, Marburg, Germany;(4) Mikrobiologisches Institut, Eidgenössische Technische Hochschule, ETH-Zentrum, Zürich, Switzerland;(5) Department of Microbiology, University of Melbourne, 3052 Parkville, Victoria, Australia |
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Abstract: | An anaerobic bacterium was isolated from a polluted sediment, with succinate and yeast extract as carbon and energy sources. The new strain was Gram-positive, the cells were coccal shaped, the mol% G+C content of the genomic DNA was 29, and the peptidoglycan was of the L-ornithine-D-glutamic acid type. Comparative sequence analysis of the 16S rRNA gene showed the new strain to belong to the genus Peptostreptococcus. Succinate, fumarate, pyruvate, 3-hydroxybutyrate and lysine supported growth. Succinate was degraded to propionate and presumably CO2, with a stoichiometric cell yield. Key enzymes of the methylmalonyl-CoA decarboxylase pathway were present. The methylmalonyl-CoA decarboxylase activity was avidin-sensitive and sodium dependent, and about 5 mM Na+ was required for maximal activity. Whole cells, however, required at least 50 mM sodium for maximal succinate decarboxylation activity and to support the maximum growth rate. Sodium-dependent energy conservation coupled to succinate decarboxylation is shown for the first time to occur in a bacterium belonging to the group of Gram-positive bacteria containing the peptostreptococci and their relatives. |
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Keywords: | anaerobic degradation energetics methylmalonyl-CoA decarboxylase Peptostreptococcus sodium-dependent energy conservation succinate |
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