Regeneration of plants from leaflet explants of tissue culture raised safed siris (Albizia procera) |
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| Authors: | Kumar Sandeep Sarkar AK Kunhikannan C |
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| Institution: | (1) Genetics and Plant Propagation Division, Tropical Forest Research Institute, P.0. RFRC, Mandla Road, Jabalpur (M.P.), 482 021, India;(2) Non Wood Forest Produce Division, Tropical Forest Research Institute, P.0. RFRC, Mandla Road, Jabalpur (M.P.), 482 021, India |
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| Abstract: | Shoot bud regeneration was obtained from isolated leaflets of Albizia procera cultured on MS medium with various concentrations
of 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA). The highest numbers of adventitious buds were obtained on MS medium
supplemented with 10 μM BA and 1 μM NAA. The replacement of 7 g l-1 Difco bacto agar with 2.6 g l-1 Phytagel in the medium
enhanced adventitious bud regeneration. Further, addition of 15 μM silver nitrate promoted callus-free shoot regeneration
from leaf explants. The regenerated shoot buds were elongated on MS medium containing 0.01 μM BA and 1 μM NAA. Rooting was
obtained on modified MS medium supplemented with 2 μM IBA. To our knowledge this is the first report of direct regeneration
of shoots from leaflet explants in A. procera, and should help facilitate genetic transformation in this species.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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| Keywords: | Albizia procera BA leaf explant Phytagel shoot bud regeneration root initiation |
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