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Tolerance of plastic-encapsulated Pseudomonas putida KT2440 to chemical stress
Authors:Susana Vílchez  Alan Tunnacliffe  Maximino Manzanera
Institution:(1) Institute of Biotechnology, University of Granada, Campus Fuentenueva, 18071 Granada, Spain;(2) Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK;(3) Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, UK;(4) Institute of Water Research, University of Granada, CL. Ramon y Cajal 4, 18071 Granada, Spain
Abstract:Pseudomonas putida dried in the presence of hydroxyectoine or trehalose can withstand exposure to organic solvents and therefore can be encapsulated inside plastics such as polystyrene. Here we show that P. putida in a plastic-encapsulated dried tablet exhibits remarkable tolerance to chemical stress, comparable to that of spores of Bacillus subtilis.
Keywords:Anhydrobiosis            Pseudomonas putida            Chemical stress  Hydroxyectoine  Trehalose            Bacillus subtilis
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