仔猪人工感染猪繁殖与呼吸综合征病毒后宿主细胞凋亡动态变化规律的研究

28日龄长白仔猪滴鼻人工感染猪繁殖与呼吸综合征病毒(PRRSV),于接种后8h、24h、30h、3d、5d、7d、9d、11d、14d、21d、28d、35d和42d各随机剖杀2头,取各组织器官,利用原位末端标记(TdT-mediated dUTP nick end la-beling,TUNEL)法检测PRRSV感染仔猪后所产生的细胞凋亡情况。结果表明,PRRSV在体内可诱导肺脏、淋巴结(肺门淋巴结、肠系膜淋巴结、腹股沟淋巴结)、扁桃体、脾脏、肝脏、胰脏、十二指肠、胸腺、肾脏、子宫、睾丸、附睾、大脑和小脑等组织的细胞发生凋亡,发生凋亡的靶细胞主要是各种巨噬细胞、单核细胞以及生殖细胞。感染后8h即可检测到细胞凋亡,且凋亡细胞数目随着感染时间的延长在感染后第7d达到高峰,以后逐渐减少,至感染后42d时仍能检测到少量凋亡细胞。

Study on Dynamic Changes of Apoptosis of Host Cells after the Artificial Infection of Piglets by Porcine Reproductive and Respiratory Syndrome Virus

Piglets of 28 days-old were inoculated intranasally with porcine reproductive and respiratory syndrome virus(PRRSV).At 8h,24h,30h,3d,5d,7d,9d,11d,14d,21d,28d,35d and 42d after inoculation,every two piglets were killed and tissue samples were collected.Apoptosis of host cells were identified by TUNEL method.The results showed that PRRSV infection could induce the apoptosis of lung,lymph nodes(including pulmonary portal lymph nodes?mesenteric lymph nodes?inguinal lymph nodes),tonsil,spleen,heart,liver,pancreas,duodenum,thymus,kidney,uterus,testicle,cerebrum and cerebellum,etc.The apoptotic cells were mainly macrophages,monocytes and germ cells.The apoptosis of host cells could be detected at 8h after infection,and the number of apoptotic cells increased along with the extending of the infection time,and peaked on 7d,then decreased.A small amount of apoptotic cells could also be detected until 42d after infection.