| 杆状病毒凋亡抑制基因的稳定转化及抗凋亡昆虫细胞系的构建 |
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| 引用本文： | 李小峰,齐义鹏,朱应,王业富.杆状病毒凋亡抑制基因的稳定转化及抗凋亡昆虫细胞系的构建[J].昆虫学报,1999,42(2):120-125. |
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| 作者姓名： | 李小峰 齐义鹏 朱应 王业富 |
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| 作者单位： | 武汉大学生命科学学院,武汉,430072 |
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| 基金项目： | 国家科委“九五”攻关项目 |
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| 摘 要： | 构建了新霉素抗性基因为筛选标记的带有凋亡抑制基因p35的重组质粒p35IE1Neo, 转化Sf9细胞后, 经G418筛选得到含有p35IE1Neo的Sf9细胞, 克隆化培养后命名为Sf9-35。PCR检测表明, Sf9-35细胞的染色体DNA上有p35基因的扩增带。经放线菌素D处理后的细胞核酸电泳和TdT介导bio-DUTP缺口末端标记(TUNEL)试剂盒检测, 证实Sf9-35具有抗凋亡特性。
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| 关 键 词： | 抗凋亡 昆虫细胞 p35基因 |
STABLE TRANSFORMATION WITH ANTI-APOPTOTIC GENE OF BACULOVIRUS AND CONSTRUCTION OF ANTI-APOPTOTIC INSECT CELL LINE |
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| Li Xiaofeng,Qi Yipeng,Zhu Ying,Wang Yefu.STABLE TRANSFORMATION WITH ANTI-APOPTOTIC GENE OF BACULOVIRUS AND CONSTRUCTION OF ANTI-APOPTOTIC INSECT CELL LINE[J].Acta Entomologica Sinica,1999,42(2):120-125. |
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| Authors: | Li Xiaofeng Qi Yipeng Zhu Ying Wang Yefu |
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| Abstract: | A transfer vector p35IE1Neo containing anti apoptotic p35 gene and neomycin resistant gene(as selection marker) was constructed. After transfecting Sf9 cells and G418 screening, cells transformed with p35IE1Neo were obtained. One clone was picked and named Sf9 35. By PCR detection, it was found that the genome of Sf9 35 cells contain p35 gene. After actinomycin D treatment and cellular DNA electrophoresis and detection of TUNEL(TdT mediated bio dUTP nick endlabeling), Sf9 35 cells were found to be resistant to apoptosis induced by actinomycin D. |
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| Keywords: | anti apoptosis insect cells p35 gene |
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