Neomycin- and spectinomycin-resistance replacement vectors for Bacillus subtilis |
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| Authors: | Vasant K Charya Edward I Amayaa Patrick J Piggota |
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| Institution: | Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán and Centro de Referencias para Lactobacilos (CERELA), Chacabuco 145, 4000 Tucumán, Argentina;Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain |
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| Abstract: | A plasmid is described for Bacillus subtilis that facilitates replacement of the widely used neomycin resistance gene (neo) with a spectinomycin resistance (spcE) gene. A second plasmid is described that facilitates replacement of spcS, associated with mini-Tn10 mutagenesis in B. subtilis, with neo. These plasmids can also function as integrative vectors for B. subtilis. They expand the scope of strain construction and gene analysis in B. subtilis. |
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| Keywords: | Leuconostoc oenos Proteolytic activity White wine |
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