NMR solution structure of the isolated Apo Pin1 WW domain: comparison to the x-ray crystal structures of Pin1. |
| |
Authors: | Jennifer A Kowalski Kai Liu Jeffery W Kelly |
| |
Institution: | Department of Chemistry, Skaggs Institute of Chemical Biology, The Scripps Research Institute, BCC265 10550 N. Torrey Pines Road, La Jolla, CA 92037. |
| |
Abstract: | The NMR solution structure of the isolated Apo Pin1 WW domain (6-39) reveals that it adopts a twisted three-stranded antiparallel beta-sheet conformation, very similar to the structure exhibited by the crystal of this domain in the context of the two domain Pin1 protein. While the B factors in the apo x-ray crystal structure indicate that loop 1 and loop 2 are conformationally well defined, the solution NMR data suggest that loop 1 is quite flexible, at least in the absence of the ligand. The NMR chemical shift and nuclear Overhauser effect pattern exhibited by the 6-39 Pin1 WW domain has proven to be diagnostic for demonstrating that single site variants of this domain adopt a normally folded structure. Knowledge of this type is critical before embarking on time-consuming kinetic and thermodynamic studies required for a detailed understanding of beta-sheet folding. |
| |
Keywords: | WW domain NMR structure Pin1 protein folding structural comparison |
|
|