Cytological changes during cell wall sac formation in mulberry idioblasts |
| |
Authors: | Yukio Sugimura Ikuro Nitta |
| |
Institution: | (1) Graduate School of Science and Technology, Kyoto Institute of Technology, Kyoto |
| |
Abstract: | Summary. When calcium carbonate crystals are formed in mulberry (Morus abla) idioblasts, they are deposited in newly formed cell wall sacs. The initial cytological events leading to cell wall sac formation
were observed in the distal end of young idioblasts and tentatively categorized into four stages. The first indication of
formation was the separation of the innermost cell wall layer from the cell wall, which is followed by the deposition of egg-shaped
polysaccharide on the inner cell wall surface. The size of the deposit area increased, while the thickness of the cell wall
significantly decreased during the next stage. Finally, the condensed cellulosic lamella was invaginated into the deposition
area, resulting in the formation of an elongated cell wall sac. The internal cell wall sac was composed of numerous fibers
with different morphologies. Application of gelatin-methenamine-silver staining allowed us to observe the spatial distribution
of cellulosic polysaccharides as electron-dense images.
Correspondence and reprints: Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo,
Kyoto 606-8585, Japan. |
| |
Keywords: | : Calcium carbonate deposition Cell wall sac Idioblast Morus alba Cytochemistry Polysaccharide |
本文献已被 PubMed SpringerLink 等数据库收录! |
|